Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.
Identifieur interne : 000013 ( Main/Exploration ); précédent : 000012; suivant : 000014Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.
Auteurs : Emmanuel Bréard [France] ; Corinne Sailleau ; Hervé Coupier ; Karine Mure-Ravaud ; Saliha Hammoumi ; Bernard Gicquel ; Chris Hamblin ; Philippe Dubourget ; Stéphan ZientaraSource :
- Veterinary research [ 0928-4249 ]
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (composition chimique), Alignement de séquences (médecine vétérinaire), Animaux (MeSH), Données de séquences moléculaires (MeSH), Embryon de poulet (MeSH), Fièvre catarrhale du mouton (diagnostic), Fièvre catarrhale du mouton (prévention et contrôle), Fièvre catarrhale du mouton (virologie), Fièvre catarrhale du mouton (épidémiologie), Marqueurs génétiques (MeSH), Ovis (MeSH), RT-PCR (médecine vétérinaire), RT-PCR (méthodes), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Sérotypie (médecine vétérinaire), Tests de neutralisation (médecine vétérinaire), Vaccination (médecine vétérinaire), Vaccins antiviraux (génétique), Variation génétique (MeSH), Virus de la langue bleue (génétique), Virus de la langue bleue (immunologie).
- MESH :
- analyse : ARN viral.
- composition chimique : ARN viral.
- diagnostic : Fièvre catarrhale du mouton.
- génétique : Vaccins antiviraux, Virus de la langue bleue.
- immunologie : Virus de la langue bleue.
- médecine vétérinaire : Alignement de séquences, RT-PCR, Sérotypie, Tests de neutralisation, Vaccination.
- méthodes : RT-PCR.
- prévention et contrôle : Fièvre catarrhale du mouton.
- virologie : Fièvre catarrhale du mouton.
- épidémiologie : Fièvre catarrhale du mouton.
- Animaux, Données de séquences moléculaires, Embryon de poulet, Marqueurs génétiques, Ovis, Séquence d'acides aminés, Séquence nucléotidique, Variation génétique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Base Sequence (MeSH), Bluetongue (diagnosis), Bluetongue (epidemiology), Bluetongue (prevention & control), Bluetongue (virology), Bluetongue virus (genetics), Bluetongue virus (immunology), Chick Embryo (MeSH), Genetic Markers (MeSH), Genetic Variation (MeSH), Molecular Sequence Data (MeSH), Neutralization Tests (veterinary), RNA, Viral (analysis), RNA, Viral (chemistry), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Sequence Alignment (veterinary), Serotyping (veterinary), Sheep (MeSH), Vaccination (veterinary), Viral Vaccines (genetics).
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : RNA, Viral.
- chemical , genetics : Viral Vaccines.
- chemical : Genetic Markers.
- diagnosis : Bluetongue.
- epidemiology : Bluetongue.
- genetics : Bluetongue virus.
- immunology : Bluetongue virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- prevention & control : Bluetongue.
- veterinary : Neutralization Tests, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Serotyping, Vaccination.
- virology : Bluetongue.
- Amino Acid Sequence, Animals, Base Sequence, Chick Embryo, Genetic Variation, Molecular Sequence Data, Sheep.
Abstract
Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.
DOI: 10.1051/vetres:2003036
PubMed: 14746772
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000811
- to stream PubMed, to step Curation: 000807
- to stream PubMed, to step Checkpoint: 000806
- to stream Main, to step Merge: 000013
- to stream Main, to step Curation: 000013
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.</title><author><name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Bréard">Emmanuel Bréard</name><affiliation wicri:level="3"><nlm:affiliation>UMR 1161 AFSSA-INRA-ENVA, Agence Française de Sécurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort, France. e.breard@afssa.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1161 AFSSA-INRA-ENVA, Agence Française de Sécurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Maisons-Alfort</settlement></placeName></affiliation></author><author><name sortKey="Sailleau, Corinne" sort="Sailleau, Corinne" uniqKey="Sailleau C" first="Corinne" last="Sailleau">Corinne Sailleau</name></author><author><name sortKey="Coupier, Herve" sort="Coupier, Herve" uniqKey="Coupier H" first="Hervé" last="Coupier">Hervé Coupier</name></author><author><name sortKey="Mure Ravaud, Karine" sort="Mure Ravaud, Karine" uniqKey="Mure Ravaud K" first="Karine" last="Mure-Ravaud">Karine Mure-Ravaud</name></author><author><name sortKey="Hammoumi, Saliha" sort="Hammoumi, Saliha" uniqKey="Hammoumi S" first="Saliha" last="Hammoumi">Saliha Hammoumi</name></author><author><name sortKey="Gicquel, Bernard" sort="Gicquel, Bernard" uniqKey="Gicquel B" first="Bernard" last="Gicquel">Bernard Gicquel</name></author><author><name sortKey="Hamblin, Chris" sort="Hamblin, Chris" uniqKey="Hamblin C" first="Chris" last="Hamblin">Chris Hamblin</name></author><author><name sortKey="Dubourget, Philippe" sort="Dubourget, Philippe" uniqKey="Dubourget P" first="Philippe" last="Dubourget">Philippe Dubourget</name></author><author><name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003 Nov-Dec</date><idno type="RBID">pubmed:14746772</idno><idno type="pmid">14746772</idno><idno type="doi">10.1051/vetres:2003036</idno><idno type="wicri:Area/PubMed/Corpus">000811</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000811</idno><idno type="wicri:Area/PubMed/Curation">000807</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000807</idno><idno type="wicri:Area/PubMed/Checkpoint">000806</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000806</idno><idno type="wicri:Area/Main/Merge">000013</idno><idno type="wicri:Area/Main/Curation">000013</idno><idno type="wicri:Area/Main/Exploration">000013</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.</title><author><name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Bréard">Emmanuel Bréard</name><affiliation wicri:level="3"><nlm:affiliation>UMR 1161 AFSSA-INRA-ENVA, Agence Française de Sécurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort, France. e.breard@afssa.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1161 AFSSA-INRA-ENVA, Agence Française de Sécurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Maisons-Alfort</settlement></placeName></affiliation></author><author><name sortKey="Sailleau, Corinne" sort="Sailleau, Corinne" uniqKey="Sailleau C" first="Corinne" last="Sailleau">Corinne Sailleau</name></author><author><name sortKey="Coupier, Herve" sort="Coupier, Herve" uniqKey="Coupier H" first="Hervé" last="Coupier">Hervé Coupier</name></author><author><name sortKey="Mure Ravaud, Karine" sort="Mure Ravaud, Karine" uniqKey="Mure Ravaud K" first="Karine" last="Mure-Ravaud">Karine Mure-Ravaud</name></author><author><name sortKey="Hammoumi, Saliha" sort="Hammoumi, Saliha" uniqKey="Hammoumi S" first="Saliha" last="Hammoumi">Saliha Hammoumi</name></author><author><name sortKey="Gicquel, Bernard" sort="Gicquel, Bernard" uniqKey="Gicquel B" first="Bernard" last="Gicquel">Bernard Gicquel</name></author><author><name sortKey="Hamblin, Chris" sort="Hamblin, Chris" uniqKey="Hamblin C" first="Chris" last="Hamblin">Chris Hamblin</name></author><author><name sortKey="Dubourget, Philippe" sort="Dubourget, Philippe" uniqKey="Dubourget P" first="Philippe" last="Dubourget">Philippe Dubourget</name></author><author><name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name></author></analytic><series><title level="j">Veterinary research</title><idno type="ISSN">0928-4249</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Animals (MeSH)</term><term>Base Sequence (MeSH)</term><term>Bluetongue (diagnosis)</term><term>Bluetongue (epidemiology)</term><term>Bluetongue (prevention & control)</term><term>Bluetongue (virology)</term><term>Bluetongue virus (genetics)</term><term>Bluetongue virus (immunology)</term><term>Chick Embryo (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Neutralization Tests (veterinary)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (chemistry)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Sequence Alignment (veterinary)</term><term>Serotyping (veterinary)</term><term>Sheep (MeSH)</term><term>Vaccination (veterinary)</term><term>Viral Vaccines (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>ARN viral (composition chimique)</term><term>Alignement de séquences (médecine vétérinaire)</term><term>Animaux (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Embryon de poulet (MeSH)</term><term>Fièvre catarrhale du mouton (diagnostic)</term><term>Fièvre catarrhale du mouton (prévention et contrôle)</term><term>Fièvre catarrhale du mouton (virologie)</term><term>Fièvre catarrhale du mouton (épidémiologie)</term><term>Marqueurs génétiques (MeSH)</term><term>Ovis (MeSH)</term><term>RT-PCR (médecine vétérinaire)</term><term>RT-PCR (méthodes)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Sérotypie (médecine vétérinaire)</term><term>Tests de neutralisation (médecine vétérinaire)</term><term>Vaccination (médecine vétérinaire)</term><term>Vaccins antiviraux (génétique)</term><term>Variation génétique (MeSH)</term><term>Virus de la langue bleue (génétique)</term><term>Virus de la langue bleue (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bluetongue</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Fièvre catarrhale du mouton</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Bluetongue</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bluetongue virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Vaccins antiviraux</term><term>Virus de la langue bleue</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la langue bleue</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Bluetongue virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Alignement de séquences</term><term>RT-PCR</term><term>Sérotypie</term><term>Tests de neutralisation</term><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Bluetongue</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Fièvre catarrhale du mouton</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Neutralization Tests</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Serotyping</term><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fièvre catarrhale du mouton</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bluetongue</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Fièvre catarrhale du mouton</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Chick Embryo</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Sheep</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Embryon de poulet</term><term>Marqueurs génétiques</term><term>Ovis</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Maisons-Alfort</li></settlement></list><tree><noCountry><name sortKey="Coupier, Herve" sort="Coupier, Herve" uniqKey="Coupier H" first="Hervé" last="Coupier">Hervé Coupier</name><name sortKey="Dubourget, Philippe" sort="Dubourget, Philippe" uniqKey="Dubourget P" first="Philippe" last="Dubourget">Philippe Dubourget</name><name sortKey="Gicquel, Bernard" sort="Gicquel, Bernard" uniqKey="Gicquel B" first="Bernard" last="Gicquel">Bernard Gicquel</name><name sortKey="Hamblin, Chris" sort="Hamblin, Chris" uniqKey="Hamblin C" first="Chris" last="Hamblin">Chris Hamblin</name><name sortKey="Hammoumi, Saliha" sort="Hammoumi, Saliha" uniqKey="Hammoumi S" first="Saliha" last="Hammoumi">Saliha Hammoumi</name><name sortKey="Mure Ravaud, Karine" sort="Mure Ravaud, Karine" uniqKey="Mure Ravaud K" first="Karine" last="Mure-Ravaud">Karine Mure-Ravaud</name><name sortKey="Sailleau, Corinne" sort="Sailleau, Corinne" uniqKey="Sailleau C" first="Corinne" last="Sailleau">Corinne Sailleau</name><name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Bréard">Emmanuel Bréard</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000013 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000013 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:14746772
|texte= Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:14746772" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |